Development and validation of a SNP-based genotyping tool for pedigree establishment in Australian greenlip abalone Haliotis laevigata Donovan, 1808

To enable cumulative increases in aquaculture productivity, structured and efficient selective breeding programs are required. These contingent on the management of genetic resources within population though attainment accurate pedigree information which can be provided by DNA-based parentage analysis. This study developed a SNP panel for greenlip abalone, Haliotis laevigata, from DArTSeq data produced audit abalone (n = 336) five farms. The initial dataset consisted 15,320 SNPs. Strict filtering quality control metrics was conducted to select most informative 1,004 SNPs design DArTag™ genotyping abalone. Sixteen broodstock 1035 hatchery progeny were genotyped using panel. resulting genotypes filtered, produce 705 high performing In silico analysis subsets these (highest ranked polymorphic content) revealed that inclusion < 50 satisfactory resolve 10,000 simulated up 200 candidate parents. resolving power also assessed under levels inbreeding (0–50 %) relatedness between parents, conditions missing parental (10–50 silico. Under all inbreeding, simulated, able accurately assign pairs offspring. validate results, 16 both CERVUS APIS. Complete assigned experimental progeny, with 100 % consensus two methods used. indicates this will serve as an cost-effective tool establishment